The overall goal of our research is to understand how signals at the plasma membrane regulate the actin-based cytoskeleton, cell movement and proliferation. We are particularly interested in signal transduction mechanisms that are mediated through changes in intracellular pH, and how H+ fluxes at the plasma membrane drive physiological processes. H+ fluxes across biological membranes regulate cell proliferation, cell adhesion, cytoskeleton dynamics, vesicular trafficking, apoptosis, and bacterial and viral entry into mammalian cells. Our work focuses on H+ fluxes at the plasma membrane generated by ion exchangers, with an emphasis on the Na-H exchanger, NHE1.
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Selected Publications
Patel, H., and Barber, D.L. (2005) A developmentally-regulated Na-H exchanger in Dictyostelium discoideum is necessary for cell polarity during chemotaxis. J. Cell Biol. 169:321-239.
Denker, S.P. and Barber, D..L. (2002) Cell migration requires both ion translocation and cytoskeletal anchoring by the Na-H exchanger NHE1. J. Cell Biol. 159:1087-1096.
Denker, S.P., Huang, D.C., Orlowsky, J., Furthmayr, H., and Barber, D.L. (2000) Direct binding the Na-H exchanger NHE1 to ERM proteins regulates the cortical cytoskeleton and cell shape independently of H+ translocation. Mol. Cell 6:1425-1436.
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